Allelic and haplotypic frequencies of HLA genes in hematopoietic stem-cells donors attended in a laboratory of Paraná
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Abstract
The success of hematopoietic stem cell transplantation (HSCT) depends on efficient measures that allow the prevention of graft-versus-host disease and, especially, HLA compatibility between donor and recipient. The Laboratório de Imunogenética (LI) of the Complexo do Hospital de Clínicas of Universidade Federal do Paraná (CHC-UFPR) conducts HLA genotyping, as well as the identification of anti-HLA antibodies, before HSCT. The aim of the present study was to determine the allelic and haplotypic frequencies of HLA class I and II loci, and to determine the linkage disequilibrium among HLA genes, in related and non-related donors registered in the CHC-UFPR LI database, to assist in the search of donors, facilitating the selection process, as well as in conference of complex results in the identification of anti-HLA antibodies. Allele frequencies were calculated by direct counting. Haplotypic frequencies were inferred by the ELB algorithm and linkage disequilibrium between the genes (D') was also estimated. The allelic groups with the highest frequency of HLA class I and II genes found in this population were HLA-A*02, B*35, C*07, DRB1*07, DQB1*03 and DPB1*04. The highest frequency of HLA class I and II alleles in this population were HLA-A*02:01, B*07:02, C*04:01, DRB1*07:01, DQB1*03:01 and DPB1*04:01. The highest frequency haplotypes were A*02-B*44, A*01-B*08-C*07, A*01-B*08-DRB1*03 and DQB1*03-DPB1*04. The LI team had access to the frequencies to contribute to the understanding of the sample in their database, as well as in solving possible complex results in exams.
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